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. 2011 Feb 11;6(2):e15786. doi: 10.1371/journal.pone.0015786

Figure 2. Initial dopaminergic neurogenesis is not affected in Dkk1+/− animals.

Figure 2

(A) Immunohistochemistry for TH and TuJ1 in representative intermediate midbrain sections at E11.5. Scale bar = 50 µm (B) Quantification of the number of TH+ neurons in the VM revealed no differences in Wt and Dkk1+/− embryos (mean ± s.e.m- Wt : 46.8±10.9, N = 6; Dkk1+/− : 38±14.7, N = 5. (C) Immunohistochemistry for TH and Lmx1a in serial sections through the VM revealed no alteration in DA cells at E11.5. Scale bar = 50 µm. (D) No differences were observed in the number of Lmx1a+ cells at this stage (mean ± s.e.m- Wt : 1577±195.7, N = 3; Dkk1+/− : 1483±114.9, N = 3).