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. Author manuscript; available in PMC: 2011 Feb 12.
Published in final edited form as: Anesthesiology. 2011 Jan;114(1):49–57. doi: 10.1097/ALN.0b013e318201dcb3

Fig. 4.

Fig. 4

TAT-Pep5 decreases isoflurane-mediated reduction in dendritic spines in hippocampal slices. Hippocampi were dissected from postnatal day (PND4-7) mouse pups and 400 μm slices were exposed to isoflurane with or without pretreatment with TAT-Pep5 (15 min; 10 μM). Images represented in 4X magnification are as follows: basal (CTRL, A), isoflurane (ISO, B), Pep5 CTRL (C), Pep5 ISO (D). 60X magnification images are as follows: CTRL (E), ISO (F), Pep5 CTRL (G), and Pep5 ISO (H). Isoflurane exposure significantly (n = 4, # p = 0.0041 vs. basal) reduced drebrin immunofluorescence in hippocampal slice cultures; TAT-Pep5 significantly (n = 4, * p = 0.0007 vs. isoflurane) attenuated the isoflurane-mediated reduction in drebrin expression. Quantitation of the data is represented in panel I. Drebrin expression (green pixels) was normalized to DAPI (blue pixels). Error bars, standard error of the mean (s.e.m.).