Table 1.
Protein | Cell line | Observed effect | Reference |
anti-N-WASP immunoreagents | Cos-7 | Strong reduction of transferrin-endocytosis | Kessels 200263 |
anti-EGFR phospho-Tyr845 IgG | A431 cells | Marked decrease in cell stimulation-dependent phosphorylation of Stat3 | Sato 200374 |
anti-survivin mAb/pAb | HeLa | Microtubule defects, formation of multipolar mitotic spindles, appearance of multinucleated cells | Fortugno 2002, Fortugno 200364,75 |
c-Fos or c-Jun antibody | quiescent PC12 | Completely blocked neurite formation | Gil 200465 |
Anti-actin IgG | BHK | Depolymerization of cytoskeleton fibers | Dalkara 200476 |
Anti Tubulin IgG | HeLa | ||
Dynamitin β-galactosidase as control | Axons of Sensory neurons (primary cells) | Retrograde transport (from axon to cell body) prevented | Heerssen 200477 |
Monoclonal anti-lamin antibody Avidin-Alexa488 | Human U87 | Localization at lamin site, formation of micronuclei (change in morphology assumed to be due to partial blockage of lamin function) if high amounts of antibody had been taken up | Didenko 200578 |
Constitutively active recombinant MEK-1 | OK | Increased activation of endogenous ERK, translocation of PKCα to the membrane fraction, increased phosphorylation of the Na+-K+- ATPase α1 subunit, inhibition of Na+ K+-ATPase activity | Khundmiri 200566 |
Cif | HeLa | Enlargement of the cells and formation of actin stress fibers, G2-arrest | Taieb 200667 |
Crkl | Splenocytes (primary cells) | Significant (p < 0.00003) increase in Stat5b DNA binding ability | Laloraya 200679 |
Rabbit anti-Cpn0585 or rabbit anti-Chlamydia EB (elementary body) antibodies | HEp-2 cells infected with C. pneumoniae | Used for visualization (cells fixed afterwards) | Cortes 200780 |
Purified human TTase FITC-tagged antibody for determination of transfection efficiency | TTase−/− mouse LECs (lens epithelial cells) | Increased TTase activity in TTase−/− cells | Lofgren 200869 |
β-galactosidase as a negative control | Detoxification of H2O2 in TTase loaded TTase−/− cells | ||
Restriction endonucleases | OVCAR | Genome digestion | Geel 200981 |
SKOV-3 | |||
NAD+ glycohydrolase | Neurons (primary cells) | Decrease in cytosolic NAD+ | Alano 201071 |
ATPase, adenosine triphosphatase; Cif, cycle inhibiting factor; Crkl, v-crk sarcoma virus CT10 oncogene homolog (avian)-like; EGFR, epidermal growth factor receptor; ERK, extracellular signal-regulated kinase; FITC, fluorescein isothiocyanate; mAb, monoclonal antibody; MEK, mitogen-activated protein kinase/extracellular signal-regulated kinase kinase; NAD+, nicotinamide adenine dinucleotide; pAb, polyclonal antibody; PKCα, protein kinase Cα; Stat, signal transducers and activators of transcription; TTase, thioltransferase; WASP, Wiskott-Aldrich syndrome protein.