Table 2.

Transbodies

Construct	Linkage/Production method	PTD	Cell lines	Reference
Fab	Conjugate	HIV1-TAT(37–62)	A431 breast carcinoma cells	Anderson 1993139
F(ab′)2	Conjugate	HIV1 TAT(37–72)	Bovine chromaffin cells	Stein 1999142
whole IgG	Conjugate	KGEGAAVLLPVLLAAPG (“MTS“)	NIH 3T3 cells	Zhao 2001140
whole IgG	Conjugate	R68	HeLa cells	Chen and Erlanger 2002143
			MCF-7 cells
			SK-BR-3 cells
			murine lung enthothelial line
			NIH 3T3 cells
scFv	Conjugate	HIV1 TAT (44–57)	HEK293 mice (injection)	Niesner 2002141
mAbs, pAbs	Conjugate	KGEGAAVLLPVLLAAPG (“MTS“)	Human lymphoma T cells	Zhao 2003144
scFv	Genetic fusion, C-terminal	HIV1 TAT(47–57) YGRKKRRQRRR	BMMC, RBL, MCF7	Cohen-Saidon 2003145
scFv, radiolabeled	Genetic fusion, 5′-terminal of scFv, produced as inclusion body	HIV1 TAT(47–57) YGRKKRRQRRR	Mice	Nakajima 2004146
mAb	Conjugate	HIV1 TAT(47–57) YGRKKRRQRRR	MIN6 β cells	Ohara-Imaizumi 2004147
scFv	Fusion	MTS	293T, BT-474 and PyVmT cells	Shin 2005148
Fab radiolabeled	Conjugate	HIV1 Tat protein (positions 48–60)	HeLa, rats	Kameyama 2006149
		Antennapedia (positions 43–58)
		HIV1 Rev protein (positions 34–50)
Fab-Rev radiolabeled	Conjugate	HIV1 REV peptide (positions 34–50) (TRQAR RNRRR RWRER QRGC)	HeLa, rats	Kameyama 2006150
IgG	Conjugate	R68	NIH 3T3	Chen 2006151
Scfv	Genetic fusion, produced as inclusion body	HIV1 TAT(47–57)	Jurkat T cells	Theisen 2006152
		YGRKKRRQRRR
Fab radiolabeled	Conjugate	TAT (GRKKRRQRRRPPQ-C-amide), REV (TRQARRNRRRRWRERQR-GC-amide) and ANP (RQIKIWFQNRRMKWKK-GC-amide)	HeLa	Kameyama 2007153
mAb	Conjugate	GRKKRRQRRRPPQGYGC	MDA-MB-468	Hu 2007154
scFv	Antennapedia genetic fusion, C-terminal, immunoaffinity purification	FlRQIKIWFQNRRMKWKK	HCT-116, MRC-5	Avignolo 2008155
scFv	Genetic fusion	Penetratin	MDCK cells	Poungpair 2010156

Fab, fragment antigen-binding; mAbs, monoclonal antibodies; MTS, membrane transport sequence; pAbs, polyclonal antibodies; PTD, protein transduction domain; scFv, single chain fragment variable.