Figure 1.

A model for the vacuole import and degradation pathway. During glucose starvation, cargo proteins destined for the Vid pathway associate with the TORC1 complex. After the addition of glucose, Tor1p dissociates from this complex. This facilitates the cargo proteins to be phosphorylated. Thereafter, Tco89p functions to import the cargo proteins into the Vid vesicles. The Vid vesicles merge with endosomes to deliver the contents to the vacuole for degradation. Retrograde vesicles form from the vacuole membrane as means to maintain the size of the vacuole.