Figure 1.

(A–D) Immunofluorescence assay of PfeIF4E (B) and PfeIF4A (C) respectively in fixed parasite cultures showing co-localization (D). (E) Effect of dsRNA on in vitro growth of the parasite. The relative growth of unsynchronized cultures at various time-points after treatment with dsRNA was measured and plotted. The results are represented as percent inhibition of the growth compared to control cultures. The bars indicate standard deviation. The curves are as follows: (a) cultures treated with PfeIF4A and PfeIF4E dsRNA; (b) cultures treated with PfeIF4A dsRNA; (c) cultures treated with PfeIF4E dsRNA; and (d) cultures treated with control GFP dsRNA.