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. Author manuscript; available in PMC: 2011 Feb 12.
Published in final edited form as: J Gerontol A Biol Sci Med Sci. 2008 Dec;63(12):1277–1288. doi: 10.1093/gerona/63.12.1277

Figure 1.

Figure 1

Electropherograms of mitochondrial proteins labeled with Alexa 488 hydrazide. A, Young, fast-twitch. B, Old, fast-twitch. C, Young, slow-twitch. D, Old, slow-twitch. Separation, −570 V/cm; hydrodynamic injection, 11 kPa, 4 seconds; sieving matrix, 20 mM Tris, 20 mM tricine, 8% dextran (462 kD), 0.5% sodium dodecyl sulfate, pH 8. The samples were analyzed in triplicate. Capillary conditioning, fluorescence labeling, and detection are described in Materials and Methods. A.U. = arbitrary units.