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Bmi-1 modulates Akt/GSK3β/Snail pathway. (A) Cell extracts were analyzed by immunoblotting with antibodies against the indicated proteins. Anti-α-Tubulin was used as the loading control. (B) Immunofluorescence identified the effect of Bmi-1 on the subcellular localization of Snail proteins (Green) in MCF-10A cells.
