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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Oct 16;1812(10):1301–1313. doi: 10.1016/j.bbadis.2010.10.004

Fig. 1. ErbB2 expression in rat kidney tissue.

Fig. 1

Representative immunohistochemical staining for ErbB2 detection in kidney cortical sections of Sprague-Dawley rat. Original magnifications are 40× (b) and 100× (c). Scale bars are 50 μm. Negative control (a) (stained with secondary antibodies in the absence of primary antibodies) at 40× is also shown. The kidney was fixed for 24 hours in Zinc Formalin and processed for paraffin embedding as described previously [23, 25]. The kidney sections were cut, dried and deparaffinized for subsequent labeled streptavidin-biotin immunohistochemistry. Tissue sections were incubated for 90 minutes in a 1:250 concentration of anti-ErbB2 (Cat. #ab2428; Abcam Inc., Cambridge, MA).