Figure 5.

Function of the AR2 site in anaerobic induction. (A) FY23 cells were transformed with the C6 plasmid, which contains the AR2 site on a 46 bp fragment, or, in descending order, by plasmid M2, M4 or M6. The latter three plasmids are derivatives of C6 generated by introduction of the indicated nucleotide substitutions within the AR2 site (M2, AAAAATTaTTGA; M4, AAAAAccGTTGA; M6, gtctcTTGTTGA). Transformed cells were grown under anaerobic and aerobic conditions and analyzed for β-galactosidase as described in Figure 2. (B) FY23 cells were transformed with the plasmid AR2/MEL1 or the MEL1 vector. Transformants were grown under anaerobic and aerobic conditions and analyzed for α-galactosidase as described in Materials and Methods.