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Role of AR2 sites in the full promoter. FY23 cells carrying derivatives of plasmid D1 containing nucleotide substitutions in either or both AR2 sites (AR2a and AR2b as defined in Fig. 1) and a control plasmid (S7) with normal AR2 sites were grown and analyzed as in Figure 1. All four plasmids contain identical substitutions adjacent to the AR2 sites introduced to facilitate plasmid construction.
