Fig. 1.

Expression of PTPγ in the early spinal cord. Expression of the chick PTPγ gene was detected with in situ hybridization at stages HH18 (F), HH20 (L), HH22 (R, S) and HH26–27 (E5) (T) and compared to localisation of neurofilament-associated protein 3A10 (A, G, and M), Lim homeodomain factors Isl1/2 (B, H, N), Lim1/2 (C, I, O), Lim3 (D, J, P) and Mnr2/HB9 (E, K, and Q). Early dorsal interneurons express both Lim1/2 and PTPγ (white arrowheads; C and F). Panel S is an enlarged, rotated version of R to compare with expression at HH26–27 (T). Square brackets demarcate the pMN domain (F, L, R, and S). In S and T: rp, roof plate; fp, floor plate; the p2 and p3 regions are demarcated in S; stars indicate motor horns Scale bar = 50 μm (A–S), 100 μm (T).