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Analysis of the presence of SpoIISA in the membrane (M) and cytosolic fractions (C) of B. subtilis cells grown in DSM medium. The first six lanes designate PY79 wild-type cells at different h of growth where initiation of sporulation corresponds to 5 h of growth. The seventh and the eighth lanes designate the IB1235 strain (spoIIS null mutant) harvested after 5 h of growth. C-IISA designates a lane with 5 ng of purified cytosolic part of SpoIISA.
