Table 1.
Bacterial strains and plasmids used in this study.
| Strains/plasmids | Description | Source/reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| MM294 | F-, endA-1, hsdR-17, (rk-,mk), supE44, thi-1,recA+ | Backman et al. (1976) |
| IB 805 | MM294 transformed with pACYC184 | This study |
| IB 1058 | MM294 transformed with pACYC-IISAB | This study |
| B. subtilis | ||
| PY79 | Prototrophic derivative of B. subtilis 168 | Youngman et al. (1984) |
| MO1099 | amy::erm | Guérout-Fleury et al. (1996) |
| IB220 | spo0A::kan | Schmeisser et al. (2000) |
| IB1073 | MO1099 amy::Pxyl-gfp-spc | Pavlendová et al. (2010) |
| IB1235 | PY79 spoIIS::kan | This study |
| IB1237 | MO1099 amy::Pxyl-NIISA-gfp-spc | This study |
| Plasmids | ||
| pUS19 | pUC19 with SpR | Benson and Haldenwang (1993) |
| pUS19-FRIIS | pUS19 with flanking regions upstream of spoIISA and downstream of spoIISB | This study |
| pUS19-ΔIIS | pUS19-FRIIS with kan | This study |
| pSG1154 | bla amyE3′spc Pxyl-gfp amyE5′ | Lewis and Marston (1999) |
| pSG1154-NIISA | bla amyE3′spc Pxyl-sequence of 86 N-terminal residues of SpoIISA-gfp amyE5′ | This study |
| pACYC184 | CmR TcR | Chang and Cohen, 1978 |
| pACYC-IISAB | CmR TcRspoIIS | This study |