Figure 10.

Confocal microscopy images of HeLa cells showing the subcellular distribution of calcein fluorescence. The cells were treated with (a) 2.0 mg mL⁻¹ calcein, (b) both 2.0 mg mL⁻¹ calcein and 1.0 mg mL⁻¹ poly(l-lysine iso-phthalamide), (c) both 2.0 mg mL⁻¹ calcein and 0.025 mg mL⁻¹ PP-75, and (d) both 2.0 mg mL⁻¹ calcein and 0.1 mg mL⁻¹ PP-75. Images were acquired with an Olympus FluoView™ FV300 confocal unit (×60 magnification) at 3.5 h after 30 min of uptake.