TABLE 1.
METHOD VALIDATION PARAMETERS
| Parameters | Method 1 | Method 2 | |||
|---|---|---|---|---|---|
| AM | CE | AM | CE | ||
| Linearity range (μg/ml) | 120-420 | 10-35 | 5-50 | 7.5-75 | |
| Correlation coefficient | 0.9988 | 0.9993 | -- | -- | |
| Regression equation (Y*) | |||||
| Slope (B) | 0.0235 | 0.0671 | -- | -- | |
| Intercept (A) | 0.0098 | 0.0152 | -- | -- | |
| Precision (%CV) | |||||
| Repeatability of sample application n=9 | 0.83 | 0.89 | 0.92 | 1.04 | |
| Limit of Detection (LOD)** | 0.0328 | 0.0648 | 1.12 | 0.45 | |
| Limit of Quantisation (LOQ)** | 0.1076 | 0.2160 | 3.75 | 1.45 | |
| Specificity | Specific | Specific | Specific | Specific | |
*
Y= A+B*C, where C is the concentration in μg/ml and Y is absorbance unit.
**
Data obtained by nine determinations.