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Induction of rhoB mRNA expression by UV irradiation is independent of c-Fos and p53. NIH 3T3 wild-type cells and c-fos–/– and p53–/– fibroblast cells were left untreated (Control) or were irradiated with UV light (40 J/m2). Thirty minutes after treatment, total RNA was isolated and the expression of rhoB mRNA was analyzed by northern blot analysis as described in Materials and Methods. The autoradiogram is shown. The relative amount of rhoB mRNA in untreated cells was set to 1.0.
