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. 2001 Feb 1;29(3):792–798. doi: 10.1093/nar/29.3.792

Figure 3.

Figure 3

Gel retardation analysis using the 0.17 kb rhoB promoter fragment. (A) An aliquot of 5 µg protein from NIH 3T3 cell extracts was analyzed for DNA binding activity to the 32P-labeled 0.17 kb rhoB promoter fragment by gel retardation as described in Materials and Methods. For competition experiments the unlabeled fragment (SComp) was added in 10-fold molar excess. As a control, an AP-1-specific oligonucleotide was added in 10- and 50-fold molar excess. The arrow points to the specific band. (B) Up to 2 h after irradiation (40 J/m2) of NIH 3T3 cells extracts were prepared and analyzed for DNA binding activity using the 32P-labeled 0.17 kb rhoB promoter fragment. (C) Extracts obtained from cells left untreated (Control) and treated with UV light (40 J/m2, 30 min) were used for supershift analysis using 1 µg ATF-2- and c-Jun/AP-1-specific antibodies.