Figure 5.

ATF-2 is associated with the 0.17 kb rhoB promoter fragment. The ³²P-labeled 0.17 kb rhoB promoter fragment was incubated with cell extracts under the same experimental conditions as used in the gel retardation analyses. Afterwards UV cross-linking and subsequent immunoprecipitation with anti-ATF-2- and c-Jun/AP-1-specific antibodies were performed as described in Materials and Methods. As a further control antibodies were inactivated by heat (5 min, 95°C) prior to use for immunoprecipitation. After washing, the amount of immunoprecipitable radioactivity was determined by scintillation counting. Data shown are means ± SD from at least three independent experiments, each performed in duplicate.