TABLE 4.
ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF R. REPENS
| Microorganisms | Pet ether (μg/disc) | Benzene (μg/disc) | Chloroform (μg/disc) | Acetone (μg/disc | Ethanol (μg/disc) | Rifampicin (μg/disc) | Miconazole (μg/disc) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 30 | 60 | 30 | 60 | 30 | 60 | 30 | 60 | 30 | 60 | 30 | 10 | |
| Bacteria | ||||||||||||
| Escherichia coli | + | + | − | − | − | + | + | ++ | ++ | +++ | +++ | NT |
| Staphylococcus pyogenes | − | − | − | − | − | + | + | ++ | ++ | +++ | +++ | NT |
| Staphylococcus aureus | − | − | − | + | + | ++ | ++ | ++ | ++ | +++ | +++ | NT |
| Pseudomonas aeruginosa | − | − | + | + | − | − | + | ++ | + | ++ | +++ | NT |
| Bacillus subtilis | − | − | − | − | − | + | ++ | ++ | ++ | ++ | +++ | NT |
| Bacillus cerus | + | + | − | − | − | + | + | + | ++ | +++ | ++ | NT |
| Fungi | ||||||||||||
| Aspergillus niger | + | ++ | − | + | + | ++ | + | ++ | ++ | +++ | NT | +++ |
| Aspergillus flavus | + | + | − | − | + | + | − | + | ++ | +++ | NT | +++ |
| Candida albicans | + | + | − | − | + | ++ | ++ | +++ | +++ | +++ | NT | +++ |
| Fusarium oxyporum | − | − | − | − | − | + | − | + | + | ++ | NT | +++ |
| Fusarium solani | − | − | − | + | − | + | − | + | + | ++ | NT | +++ |
| Trichophyton mentagrophytes | + | ++ | + | + | ++ | +++ | + | ++ | ++ | +++ | NT | +++ |
Experiments were done in triplicate. Disc diameter = 4mm. Diameter of zone of inhibition:
−
= < 4;
+
= 5-10;
++
= 11-15;
+++
= > 16;
NT = not tested. DMF had not shown any antimicrobial activity against the tested organisms.