Fig. 4.

Consumption of increasing concentrations of ethanol by SJL inbred strain and their reciprocal F1 hybrids from intercross with B6 inbred strain after two consecutive periods of abstinence in a two-bottle preference test. a–c Amount of ethanol consumed (g/kg/day) in SJL, SJLxB6 F1 and B6xSJL F1 hybrids correspondently. d–f Preference for ethanol in SJL, SJLxB6 F1 and B6xSJL F1 hybrids correspondently. g–i Total fluid intake (g/kg/day) in SJL, SJLxB6 F1 and B6xSJL F1 hybrids correspondently. n—See legends to Fig. 1. Ethanol and water intake were measured after first and after second periods of abstinence at 9, 18 and 27% concentrations of ethanol. These numbers were compared with data for experimentally naïve mice (first presentation of ethanol). *1—Statistically significant differences from initial trial (0) and trial 1 within one concentration of ethanol solution (two-way ANOVA with Post hoc Bonferroni Test). *2—Statistically significant differences from initial trial (0) and trial 2 within one concentration of ethanol solution (two-way ANOVA with Post hoc Bonferroni Test). For P values see Supplemental Tables VI, VII. R0, R1 and R2—see legends to Fig. 3