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One-dimensional 1H NMR spectra of ghrelin (a and b) and des-ghrelin (c and d), showing overlays of the aromatic and NH regions (a and c) and alkyl chain region (b and d) at 0 (red), 10 (green) and 20 mM (blue) DPC. Signals of ghrelin that shifted upon the addition of DPC are annotated.
