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. Author manuscript; available in PMC: 2011 Feb 14.
Published in final edited form as: Dev Biol. 2010 Sep 27;350(1):13–23. doi: 10.1016/j.ydbio.2010.09.016

Figure 5. mAPOBEC2 inhibits TGFβ signaling in C2C12 myoblasts.

Figure 5

A. RT-PCR analysis of mA2 and the muscle differentiation marker Skeletal-α-Actin mRNA levels during C2C12 differentiation. B. Real-time qPCR analysis of the mRNA levels of A2 and the indicated muscle markers in C2C12 cells transfected either with control (siC) or anti-A2 (siA2) siRNAs and differentiated for 2 days in DM. Numbers above the histogram bars indicate the relative decrease in mRNA levels. C, D. A2, but not AID, inhibits TGFβ signaling in C2C12 cells. Cells were transfected with the p3TP-lux reporter in combination with an empty vector (Vector) or an A2 (C) or AID (D) expressing vector. After transfection, cells were cultured with or without 5ng/ml recombinant TGF-β1 for 24 hours before harvesting for luciferase assay. Numbers above the histogram bars indicate the fold reduction in luciferase activity.