Figure 2.

Luciferase expression levels from infected erythrocytes after transfection and uptake of plasmid DNA. Parasites were initially allowed to infect erythrocytes that had been loaded with 10 µg of either linearized or circular, supercoiled pHLH-1. Subsequently, one half of the total culture volume was harvested daily for luciferase measurements and replaced either with an equal volume of culture media containing uninfected erythrocytes that had not been loaded with plasmid DNA (linear and circular, not replenished) or with erythrocytes that had been loaded with circular plasmid DNA (circular, replenished). Parasitemias were maintained at a constant level of 5–7%. Experiments were performed in triplicate; standard deviations are indicated by vertical bars.