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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Exp Hematol. 2010 Sep 18;39(1):77–86.e1-5. doi: 10.1016/j.exphem.2010.09.003

Fig. 3.

Fig. 3

MLL fusion proteins colocalize with elongating Pol II and are associated with elevated levels of H3K79 di- and tri-methylation A) ChIP-qPCR for Pol II-CTD and Ser2 and 5 phosphorylated CTD across Hoxa9 in leukemic cells lines transformed by MLL fusion proteins or E2A-HLF. Graph represents three independent experiments. The p value is shown for difference at the most 3’ site. Schematics of the Hoxa9 locus are depicted below the graphs in A and C. Red arrows indicate transcriptional start sites. Black boxes indicate exons. B) Cell viability and qPCR for gene expression after 24 hours of flavopiridol treatment. Data are normalized to U2 snRNA. C) ChIP-qPCR for Dot1L and H3K79 methylation across Hoxa9. D) qPCR for Hoxa9 and Meis1 in MV4:11 cells (express MLL-AF4) after knockdown with non-targeting control siRNA or siRNA targeting DOT1L.

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