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. 2011 Jan 12;2(2):305–314. doi: 10.1364/BOE.2.000305

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 3. — Images of hippocampal neurons labeled with SynaptoredTM C2, (a) backward TPF before electroporation, (b) forward SHG before electroporation showing localization of the depolarized (cathodic) pole; (c) backward TPF after electroporation of the same cells, (d) forward SHG after electroporation (same cells). (e) TPF signal after electroporation at the hyperpolarized pole. (f) Averaged SHG normalized to SHG when no pores are present, at depolarized (red trace), hyperpolarized (blue) poles, and border region (green), collected with NA = 0.5 condenser.