Skip to main content
. 2010 Dec 2;24(3):301–309. doi: 10.1093/protein/gzq110

Fig. 3.

Fig. 3

Purification of eIF5A(Hpu) from BL21(DE3)pLysS/pST39/eIF5A/DHS/DOHH cells. eIF5A(Hpu) was purified from E.coli cells induced with IPTG at 37°C for 4 h as described under the section Materials and methods. Chromatography on (A) DEAE Sephacel, (B) SP-Sepharose and (C) Q-Sepharose. Aliquots from each fraction were used for measurement of radioactivity by liquid scintillation counting, for protein content by the BioRad assay and for purity by SDS-PAGE and Coomassie-Blue staining of the gels. Pooled fractions are indicated by a horizontal line on top of fraction numbers of the SDS gels.