Table 2.

Cut-off point and estimates for sensitivity and specificity of RE values determined by qPCR

Gene	Area under the ROC curve	Cut-offa	Number of true carcinoma	Sensitivity	CI sensitivity	Number of true HCA	Specificity	CI specificity
1. ALDH1L1	0.941	0.6199	30	0.88	(0.69-0.96)	12	0.92	(0.62-1.00)
2. TUSC2	0.787	0.7099	23	0.68	(0.43–0.83)	12	0.92	(0.62–1.00)
3. ATF5	0.903	0.6499	23	0.68	(0.43–0.83)	12	0.92	(0.62–1.00)
4. OBSCN	0.781	0.8799	25	0.74	(0.54–0.90)	12	0.92	(0.62–1.00)
5. KLK4	0.889	0.1999	23	0.68	(0.43–0.83)	12	0.92	(0.62–1.00)
6. KLK1	0.971	0.9999	33	0.97	(0.84–1.00)	12	0.92	(0.62–1.00)
7. CHUK	0.753	0.3699	21	0.62	(0.38–0.82)	12	0.92	(0.62–1.00)
8. GFTP1	0.787	0.6099	20	0.59	(0.36–0.81)	12	0.92	(0.62–1.00)
9. DVL3	0.833	0.6899	18	0.53	(0.30–0.78)	11	0.85	(0.49–0.98)
10. PVALB	0.894	1.3899	32	0.94	(0.80–0.0.99)	12	0.92	(0.62–1.00)
11. SH3BGRL	0.842	0.6999	24	0.71	(0.49–0.87)	12	0.92	(0.62–1.00)
12. SMARCD3	0.810	0.6099	19	0.56	(0.33–0.79)	12	0.92	(0.62–1.00)
13. TBC1D1	0.873	0.3199	18	0.53	(0.30–0.78)	12	0.92	(0.62–1.00)
14. ATP1A3	0.790	0.0319	8	0.24	(0.03–0.65)	11	0.85	(0.49–0.98)
15. NUDT22	0.882	1.0199	27	0.79	(0.58–0.92)	12	0.92	(0.62–1.00)
16. TSPAN3	0.869	1.5199	25	0.74	(0.54–0.90)	12	0.92	(0.62–1.00)
17. ENAH	0.735	0.3299	13	0.38	(0.14–0.68)	12	0.92	(0.62–1.00)

LOC389833 was not included in this step (P > 0.05). CI, Confidence interval. Comparison of malignant (CCH, FTC, and FVPTC; n = 34) and HCA (n = 13). 

^aUnder this value, it was classified as malignant.