Skip to main content
. 2001 Feb 1;29(3):725–731. doi: 10.1093/nar/29.3.725

Figure 1.

Figure 1

HA epitope-tagged RNH1 vectors. The vectors contain selectable markers flanked by the L.major 5′ and 3′ dihydrofolate reductase-thymidylate synthase gene flanking regions for expression of the drug resistance marker. Amino acids are represented by standard nomenclature; black circles indicate stop codons. Sequences are not to scale. (A) pRNH1-HA has a hygromycin phosphotransferase gene as selectable marker and wild-type C.fasciculata RNH1 with six copies of the influenza HA tag cloned just upstream of the stop codon. pRNH1-Phleo has a phleomycin selectable marker but is otherwise the same as pRNH1-HA. To create pRNH1-GTG the second methionine was mutated to a valine, but is otherwise the same as pRNH1-Phleo. (B) pRNH1-STOP is pRNH1-Phleo with two stop codons created by oligonucleotide mutagenesis indicated in the inset. The amino acids altered are underlined. In the wild-type protein the underlined amino acids are TSC.