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. 2001 Feb 1;29(3):725–731. doi: 10.1093/nar/29.3.725

Figure 3.

Figure 3

Translation of sequence between the first and second ATG codons in the RNH1 ORF is not necessary for production of the nuclear isoform of RNase H1. Immunolocalization of epitope-tagged RNase H1 in cells transformed with pRNH1-STOP. rnh1Δ cells transformed with pRNH1-STOP were observed by indirect fluorescence microscopy. (A) Cells stained with DAPI to visualize the kinetoplast and nuclear DNA. (B) Immunolocalization of epitope-tagged RNase H1 with monoclonal antibodies to the HA epitope tag.