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Mutation of the second ATG codon in the RNH1 ORF to a valine codon abolishes expression of the nuclear isoform of RNase H1. Immunolocalization of epitope-tagged RNase H1 in wild-type C.fasciculata cells transformed with pRNH1-GTGH. (A) DAPI staining to visualize the kinetoplast and nuclear DNA. (B) Immunolocalization of the HA-tagged RNase H1.
