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. 2001 Feb 1;29(3):725–731. doi: 10.1093/nar/29.3.725

Figure 5.

Figure 5

RNase H activity gel analysis of epitope-tagged RNH1 constructs. Lane 1, wild-type C.fasciculata whole cell extract. The wild-type 45 and 38 kDa activities are indicated by arrows. Lane 2, rnh1Δ whole cell extract. Lane 3, whole cell extract from the rnh1Δ strain transformed with pRNH1-Phleo. Lane 4, whole cell extract from the rnh1Δ strain transformed with pRNH1-STOP showing an activity migrating at ∼46 kDa. Lane 5, whole cell extract from the rnh1Δ strain transformed with pRNH1-GTG showing an activity migrating at ∼53 kDa. The RNase H activity of the SSE1 nuclease is not visible in this exposure.