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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Ultrasound Med Biol. 2011 Jan;37(1):78–86. doi: 10.1016/j.ultrasmedbio.2010.10.002

Figure 6.

Figure 6

TEM micrographs of the kidney cortex after histotripsy treatment at the border adjacent to the homogenized region. The material in the lower right in both micrographs is the remnant of the homogenized cells, with a fragment of pyknotic nucleus remaining. The upper half of images A and B shows a cell immediately adjacent to the homogenized area appearing to show a transition between homogenate and completely intact. At 13500X magnification (C), there is a noticeable change in the morphology between the fully intact mitochondria (arrow) and the fragmented mitochondria within the homogenized region (asterisk). The homogenized area also includes keratin fragments in the lower half of the image. Other sub-cellular constituents such as endoplasmic reticulum, Golgi, lysosomes, and the cell membrane are not visible within the homogenized area. Images of the basal lamina magnified between 25000X up to 130000X (D–F) show the basal lamina (arrows) appearing intact, with no transition of fractionation. This is in spite of being within the histotripsy-treated region where the sub-cellular material within its confines appears to have been been disrupted.