Figure 4. Levels of IL-8 and VEGF in conditioned media were increased in cells expressing HPV 16E6E7C24G.
The experiment was conducted as in Figure 1. (A) The quantity of IL-8 was determined by ELISA and normalized to total protein in the conditioned media. The data represent mean ± SEM and are representative of three experiments each conducted in triplicate. p < 0.0001 for LXSN vs HPV 16E6E7; LXSN vs HPV 16E6E7C24G and p< 0.0002 for HPV 16E6E7 vs HPV 16E6E7C24G. (B) The quantity of VEGF was determined by ELISA and normalized to total protein in the conditioned media. The data represent mean ± SEM and are representative of three experiments each conducted in triplicate. p < 0.001 for LXSN vs HPV 16E6E7; LXSN vs HPV 16E6E7C24G and p< 0.0003 for HPV 16E6E7C24G vs HPV 16E6E7.