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. Author manuscript; available in PMC: 2012 Feb 9.
Published in final edited form as: J Am Chem Soc. 2011 Jan 11;133(5):1553–1562. doi: 10.1021/ja109207m

Figure 1.

Figure 1

Time-dependent inhibition of DDAH by inactivators 1-3. Time-dependent loss in DDAH (29 μM) activity (λ, solid line) is observed after incubation with 1 (300 μM) (A), 2 (1.2 mM) (B), or 3 (1 mM) (C), each in K2HPO4 buffer (100 mM), KCl (100 mM) and EDTA (0.05 mM) at pH 7.5, 25 °C. Inactivation experiments are also repeated when preceded by an incubation (1 h) of each inactivator with excess glutathione (5 mM) (ν, dashed lines); (D) Inactivated enzyme was dialyzed overnight and assayed for recovery of activity (“E“ is an enzyme-only control; “E dialysis” is enzyme-only after dialysis; “E+I” is inactivated enzyme; “E+I dialysis” is inactivated enzyme after dialysis).