Figure 2.
Time- and concentration-dependent inactivation of DDAH by 1 at pH 7.5 (A) Exponential fits to the observed inactivation of DDAH at pH 7.5 and 25 °C by different concentrations of inhibitor: 0 (○), 83 μM (λ), 140 μM (□), 330 μM (ν), 600 μM (△), 1 mM (▲), 2 mM (◇), and 3 mM (◆). (B) Concentration dependence of the pseudo-first-order kobs values give a second order rate constant of 4.8 ± 0.3 M−1 s−1 for inactivation.
