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. Author manuscript; available in PMC: 2012 Feb 9.
Published in final edited form as: J Am Chem Soc. 2011 Jan 11;133(5):1553–1562. doi: 10.1021/ja109207m

Figure 5.

Figure 5

Time- and concentration-dependent inactivation of DDAH by 1 at pH 5.0. (A) Exponential fits to the observed inactivation of DDAH (29 μM) by different concentrations of inhibitor: 0 (○), 330 μM (λ), 500 μM (□), 1 mM (ν), 1.5 mM (△), or 3 mM (▲), each in MES buffer (100 mM), KCl (100 mM), EDTA (0.05 mM) at pH 5.0, 25 °C. (B) Concentration dependence of the pseudo-first-order kobs values shows saturation kinetics and is fitted by KI (2.6 ± 0.8 mM) and kinact (0.18 ± 0.03 min−1) values for inactivation.