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Time-dependent inactivation of papain by 1 and 11. Time-dependent inactivation by 11 (1 mM, ν) is observed. No inactivation by 1 (1 mM, λ) is observed relative to a no-inactivator control (○). Prior to the assays, papain is activated by incubation with reduced L-cysteine (500 μM). Papain samples that are subsequently depeleted of free L-cysteine do not lose activity over the experimental timeframe (data not shown).
