Table 1.

PCR primers used in this study

	Forward primer (5′→3′)	Reverse primer (5′→3′)	Size (bp)	Anneal T°C
BRAF exon11	TCCCTCTCAGGCATAAGGTAA	CGAACAGTGAATATTTCCTTTGAT	313	58
BRAF exon15	TCATAATGCTTGCTCTGATAGGA	GGCCAAAAATTTAATCAGTGGA	224	58
Primer A+ D	ATTGTCATCTCGCCGTTC	CTTTCAGCATCTTCACGG	306	55
Primer B+D	GAGGGAGCTTTGGAGAAC	CTTTCAGCATCTTCACGG	266	55
Primer C+D	AAGCAAACCTGCCAGTGG	CTTTCAGCATCTTCACGG	240	55
Nested A	ACAAACTGAAGTGCAAGGCA	GCCTTGACCACTACTTTTCCAAA	151	55
Nested B	CTTTGATTTATGGAACACCG	ACTTTTCCAAATTCGCCTTC	175	53
Nested C	CCTGCCAGTGGTTATCAAGC	GGCCACCGTGGTGTACCCTG	219	58
Actin	CCTCCCTGGAGAAGAGCTACGAG	GACAGCACTGTGTTGGCGTACAG	199	62
H-ras codon 12/13	ATGACGGAATATAAGCTGGT	CCTTATAGTGGGGTCGTATT	110	52
H-ras codon 61	AGGTGGTCATTGATGGGGAG	AGGAAGCCCTCCCCGGTGCG	109	62
K-ras codon 12/13	GGCCTGCTGAAAATGACTGAA	GGTCCTGCACCAGTAATATGC	166	62
K-ras codon 61	CAGGATTCCTACAGGAAGCAAGTAG	CACAAAGAAAGCCCTCCCCA	135	62
N-rascodon 12/13	ATGACTGAGTACAAACTGGT	CTCTATGGTGGGATCATATT	111	52
N-ras codon 61	TCTTACAGAAAACAAGTGGT	GTAGAGGTTAATATCCGCAA	174	62