Figure 3.

Effects of rottlerin on the photoactivated Lonicera japonica extract-induced changes in cytoskeleton in CH27 cells. Cells were incubated with 0.1% ethanol or 100 μg/mL Lonicera japonica extracts (LJ) for 4 h and then irradiated with 0.8 J/cm² fluence dose. In rottlerin treatment, cells were pretreated with a final concentration of 10 μM rottlerin for 1 h. F-actin microfilaments in CH27 cells was visualized by TRITC-labeled phalloidin binding. To detect tubulin distribution, cells were incubated for 30 min with 250 nM Tubulin Tracker Green reagent. The specimens were observed by fluorescence microscopy (300X). Results are representative of three independent experiments.