Table 2B.

The pattern of SHM is altered in p53 −/− mice

	1	2	3	4	5	6	7	8	9	10	11
Mouse	wt1	p53−/−1	wt2	p53−/−2	wt3	p53−/−3	wt ung+ (a)	ung−/−	p53+/− ung−/−	p53−/− ung−/− 1	p53−/− ung−/− 2
A mut %(a)	32	72	33	50	30	45	36	31	34	30	30
A-G %(b)	13	22	15	25	14	23	17	15	20	13	15
			2+4+6 > 1+3+5 p4×10−4(c)				10+11 = 8+9 p0.13
A-C %(b)	11.8	22.2	9.5	13.2	7.3	11.1	7	7.9	7.4	6.8	8.7
A-T %(b)	6.5	28	8.5	11	8.7	11	11.6	7.6	6.9	10.3	6.1
			2+4+6>1+3+5 p0.06(d)				10+11 = 8+9 p0.38
C-T %(b)	5	0	10	7	7	11	10	14	14	19	18
							8+9+10+11>1+3+5+7 p8.5×10−8(g)
C-A %(b)	4.6	0	2.6	0	5.5	1.9	1.1	2.6	3.2	0.2	0.5
			2+4+6< 1+3+5 p0.01(e)				10+11 < 8 + 9 p8×10−4(e)
C-G %(b)	1.3	0	2.6	3.3	4.6	1.1	3.9	0	0	0.3	0.5
G-A %(b)	9	6	15	10	15	7	14	30	26	29	20
			2+4+6<1+3+5 p0.01(f)				10+11 = 8+9 p0.41 8+9+10+11>1+3+5+7 p3.4×10−13(g)
G-C %(b)	13.7	11.1	7.9	7.7	6.4	7.4	6	0.7	0.5	0.5	1
G-T %(b)	9.8	0	3.7	5.5	4.1	1.9	4.9	0.3	0	1.1	0
			2+4+6<1+3+5 p0.03(h)				10+11 = 8+9 p0.37
T mut % (a)	25.1	11.5	25.3	17.3	25	23.9	23.7	20.6	21.5	19.7	29.7
T-C %(b)	12	6	11	3	14	12	10	8	16	8	17
T-A %(b)	8.5	5.5	9	6.6	7.3	7.8	7.7	7.3	3.2	6.8	7.1
T-G %(b)	4.6	0	5.3	7.7	3.7	4.1	6	5.3	2.3	4.9	5.6
A/T(i)	1.26	6.5	1.29	2.81	1.25	1.85	1.52	1.48	1.82	1.49	1.02
C/G(j)	0.34	0	0.58	0.43	0.75	0.84	0.58	0.59	0.68	0.63	0.9
Age	16 weeks	16 weeks	11 weeks	11 weeks	8 weeks	8 weeks	8 weeks	8 weeks	8 weeks	8 weeks	8 weeks

^(a)Proportion of total mutations that were at A or at T

^(b)Proportion of total mutations that were the respective changes

^(c)The increase in A-G in the three p53 −/− mice (#2, 4, 6) compared with their wt littermates is highly significant (Fisher's exact test).

^(d)The increase in A-T in the three p53 −/− mice (#2, 4, 6) compared with their wt littermates is borderline signficant. The increase in A-C mutations is not significant (p0.16)

^(e)The decrease in C-A in the three p53 −/− mice (#2, 4, 6) and two p53 −/−ung−/− mice (#10, 11) compared with their p53 -wt littermates is significant

^(f)The decrease in G-A in the three p53 −/− mice (#2, 4, 6) compared with their wt littermates is significant

^(g)C-T and G-A transitions are significantly different between all ung −/− mice and all wt mice

^(h)The decrease in G-T in the three p53 −/− mice (#2, 4, 6) compared with their littermates is significant

⁽ⁱ⁾% mutations at A/% mutations at T

^(j)% mutations at C/% mutations at G

Bold: mutations in p53 −/− mice are significantly different (> or <) from p53 -wt