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. Author manuscript; available in PMC: 2012 Jan 28.
Published in final edited form as: J Mol Biol. 2010 Nov 24;405(4):972–988. doi: 10.1016/j.jmb.2010.11.028

Figure 9.

Figure 9

Cultured HepG2 cells were transiently transfected with the indicated constructs and loaded with NBD-tocopherol as described in Materials and Methods. NBD-Toc fluorescence intensity was measured in > 14 fields from each slide, averaged, and normalized to cell number. A. Expression levels of the various α-TTP variants as detected by Western blotting. B. Representative fluorescence micrographs. C. Averages and standard deviations of relative NBD-Toc fluorescence intensity measured in the indicated transfectants. Representative of two independent experiments are presented.