FIGURE 1:

Pax3 acetylation. (A) DAOY cells transfected with Pax3-pcDNA3 expression plasmid were either not treated (vehicle control) or treated with sirtinol (100 μM) or curcumin (100 μM) for 4 h. Total cell lysates were immunoprecipitated with acetyl-lysine antibody and immunoblotted with Pax3 mAb, or immunoprecipitated with Pax3 mAb and immunoblotted with acetyl lysine polyclonal antibody. Pax3 input samples were immunoblotted to confirm the presence of Pax3. (B) DAOY cells transfected with pcDNA3 vector were used as negative Pax3 controls. These cells were treated as above. Minimal endogenous Pax3 levels, depicted in the input samples, were observed. Each experiment was performed in quadruplicate.