FIGURE 6:

Identification of Neurog2 and Hes1 promoters as biological targets of acetylated Pax3. (A) EMSA binding reactions were performed with 32P-labeled double-stranded oligonucleotides, Neurog2 oligo: 5′- gacaccgtgctcggttccgggctgcgggga-3′ and Hes1oligo: 5′-cattggccgccagaccttgtgcctagcggc-3′, in the absence or presence of 100-fold molar excess of unlabeled oligonucleotide. Arrows indicate shifted and super-shifted bands. Nuclear extracts were from nontreated ND7 control cells in DMEM or from cells treated with curcumin (100 μM) or sirtinol (100 μM) for 4 h. Supershifted band intensity standardized to the control is shown in the histogram (average of three experiments ± SEM)