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. 2011 Feb 15;22(4):503–512. doi: 10.1091/mbc.E10-06-0541

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© 2011 Ichi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Regulation of Hes1 and Neurog2 expression by SIRT1 overexpression in vivo. (A) Murine SIRT1 cDNA and GFP containing plasmids were coelectroporated into the + side of chick embryonic neural tubes, and the tubes stained with anti-Hes1 and anti-Neurog2 antibodies. GFP expression was used as a positive control for electroporation. The vertical line demarcates the two sides. SIRT1 cDNA electroporation increased Hes1 and decreased Neurog2 immunostaining as shown in the inset (n = 6).