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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Microbiol. 2010 Nov 2;79(1):166–179. doi: 10.1111/j.1365-2958.2010.07436.x

Figure 5.

Figure 5

The genes for synthesis of quorum sensing autoinducers (lasI and rhlI) are necessary for rhlAB expression and swarming but adding the autoinducers does not anticipate rhlA expression. (A) lasI− and rhlI− lack swarming motility and swarming is rescued by adding the corresponding autoinducer, HSL and C4HSL respectively, to the swarming medium. (B) The lasI− mutant does not express rhlAB unless its product, HSL, is added to the medium. Nevertheless, HSL addition does not anticipate rhlAB expression relative to the wild-type. (C) The rhlI− mutant and its product, the autoinducer C4HSL, show the same effect – complementation by signal addition does not remove the delay in rhlAB expression. In both B and C, the PrhlABGFP fluorescence was normalized by GFP expression regulated by a constitutive promoter.