Fig. 4.

BAI3 fragment containing thrombospondin repeats binds to the gC1q domain of C1ql3. (A) Schematic diagram of BAI3 deletion constructs. Abbreviations are as in Fig. 1. (B) Coomassie blue–stained SDS gels of BAI3 Ig-fusion proteins expressed in HEK293 cells and purified from the medium by anti-FLAG agarose. (C) Pull-down experiments with the HA-tagged gC1q domain of C1ql3 that was immobilized on an HA-antibody column and used as an affinity matrix for the indicated purified BAI3 Ig-fusion proteins. Bound proteins were detected by immunoblot analysis for the FLAG epitopes. Data show representative experiments independently repeated three times.