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. 2011 Jan 24;108(6):2563–2568. doi: 10.1073/pnas.1012867108

Fig. 3.

Fig. 3.

TRPV4 and AQP4 colocalize in astrocytes of adult rat brain. (A–C) Single-plane confocal immunofluorescence images of large-caliber blood vessel of rat occipital cortex. Triple labeling with goat anti-AQP4 (green, A), rabbit anti-TRPV4 (red, B), and mouse anti-GFAP (blue, C) reveals astrocyte processes that are immunopositive for TRPV4 and AQP4. Triple-labeled processes appear in white in D. (Scale bar: 50 μm.) (E–G) Single-plane confocal immunofluorescence images of sagittal section of rat occipital cortex. Triple labeling with goat anti-AQP4 (green, E), rabbit anti-TRPV4 (red, F), and mouse anti-GFAP (blue, G). Triple-labeled processes appear in white in H (merged image, arrow). The triple-labeled processes (white arrows) face the pial surface and surround large-caliber blood vessels. (Scale bars: 50 μm.)