Table 1.

Summary of thermodynamic parameters for reduced apo SOD1s

apo reduced SOD*	tm, °C	ΔHvH, kcal mol-1	ΔHvH/ΔHcal	ΔCp, kcal mol-1 °C-1	ΔG,†,‡, 25 °C, kcal mol-1	ΔG,† 37 °C, kcal mol-1	ΔΔG,§37 °C, kcal mol-1
pWT	47.6 ± 0.5	50.5 ± 1.6	1.14 ± 0.15	0.72 ± 0.57	3.5 ± 0.1 4.0 ± 0.2	1.8 ± 0.1	N/A
H46R	52.6 ± 0.5	56.1 ± 4.2	0.95 ± 0.08	−0.42 ± 0.84	4.9 ± 0.1 5.1 ± 0.1	3.1 ± 0.1	+1.3
V148I	51.0 ± 1.1	58.4 ± 2.6	0.93 ± 0.04	−2.62 ± 1.19	3.6 ± 0.1	2.2 ± 0.0	+0.4
G85R	40.7 ± 0.4	46.9 ± 1.8	1.06 ± 0.36	−0.11 ± 0.34	2.1 ± 0.1	0.6 ± 0.0	−1.2
E100G	33.2 ± 1.2	47.3 ± 2.0	1.27 ± 0.06	0.79 ± 0.32	1.0 ± 0.1	−0.6 ± 0.2	−2.4
WT	46.8 ± 0.4	57.2 ± 1.1	1.49 ± 0.27	1.01 ± 0.67	3.0 ± 0.0	1.6 ± 0.0	N/A
wtH46R	52.7 ± 2.5	57.4 ± 5.4	0.76 ± 0.22	−1.59 ± 3.18	3.8 ± 0.5	2.4 ± 0.4	+0.8

N/A, not applicable.

All values are averages and standard deviations from at least three samples (Table S1), excluding WT and V148I, which are averaged over two samples.

^*All mutants are in the pWT background unless otherwise specified.

^†Values are calculated using the thermodynamic parameters obtained from the monomer two-state model and a temperature independent ΔC_p of 1.1 ± 0.1 kcal mol^-1 °C^-1 (Fig. 2 B and C).

^‡Values in italics are from monomer two-state unfolding fits of equilibrium urea chemical denaturation curves (Fig. S2 D and E).

^§ΔΔG = ΔG_(mutant)-ΔG_(pWT).