Skip to main content
. 2011 Jan 25;108(6):2228–2233. doi: 10.1073/pnas.1015287108

Fig. 7.

Fig. 7.

Verification of vOTU mutant specificity against in vivo substrates. (A) PolyUb UBE2S was incubated with wild-type and mutant vOTU and ubiquitinated species were detected by Western blotting with an anti-Ub antibody (Millipore). (B) HeLa cells were stimulated with IFNβ according to ref. 14, and total cell lysates were incubated with vOTU and mutants. ISG15 was detected by Western blotting with an anti-ISG15 antibody (Santa Cruz). WT; wild-type vOTU.

HHS Vulnerability Disclosure